A simple and effective system for the transformation and regeneration of Lycium barbarum L. has been developed.

Young stem segments from Lycium barbarum L. were infected by Agrobacterium tumefaciens harboring a vector containing neomycin phosphotransferase II (npt-II) gene derived from non-oncogenic Ti plasmid.

Calli originating from young stem segments on selective induction medium could differentiate into buds on selective differentiation medium rapidly and finally developed into whole plants. NPT-II enzyme activity assay and DNA hybridization indicated that the foreign gene had been integrated into the genome of Lycium barbarum L. and could be expressed in plants.

Wang H, Huang F, Li A, Shao Q, Niu D.
Institute of Genetics, Chinese Academy of Sciences, Beijing.

Chin J Biotechnol. 1991;7(3):185-9.

PMID: 1668526 [PubMed - indexed for MEDLINE]